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. 2026 Sep 15;17:1961230. doi: 10.3389/fpls.2026.1961230

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Copyright © 2026 Guo, Lin, Dong, Huang, Hu, Li, Tan and Shu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Panel A shows four serotonin chromatograms: blank, serotonin standard, wild type, and aas mutant, with labeled serotonin peaks. Panel B is a bar graph indicating serotonin content is significantly higher in wild type (6.66 ng/g FW) than in aas mutant (3.49 ng/g FW), with statistical significance noted by double asterisks. — Serotonin accumulation in wild-type and aas mutant seedlings upon exogenous 5-HTP feeding. (A) Representative LC-MS/MS chromatograms of serotonin standard and its content in wild-type and aas mutant plants treated with 200 μM 5-HTP for 2 days. (B) Quantification of serotonin levels. Data are presented as mean ± SD (n = 4 independent biological replicates).