TABLE 2.
ELISPOT responses obtained with peptides covering regions on HPV types 2, 6, 16, and 18 that correspond to the HPV1 sequence recognizeda
| Buffy coat sample no. | Type of T-cell response | HPV1 sequence recognized (aa) | Aligned sequences from corresponding regions on other common HPV types
|
Response | |
|---|---|---|---|---|---|
| HPV | Sequence | ||||
| 12 | CD4 | L1 (296-310) | HPV1 | TNYVGTPSGSMVSSD | Yes |
| HPV2 | HVYTSTPSGSMVSSE | No | |||
| HPV6 | SIYVNTPSGSLVSS | Yes | |||
| HPV16 | SNYFPTPSGSMVTSD | No | |||
| HPV18 | SAFFPTPSGSMVTSE | No | |||
| CD4 | E6 (126-140) | HPV1 | NRLKAKCSLCRLYAI | Yes | |
| HPV2 | NISGRWTGHCMNCGS | No | |||
| HPV6 | FIKLNCTWKGRCLHC | No | |||
| HPV16 | HNIRGRWTGRCMSCC | Yes | |||
| HPV18 | RFHNIAGHYRGQCHS | No | |||
| CD8 | L1 (351-370) | HPV1 | IS--MKNNASTTYSNANFNDFL | Yes | |
| HPV2 | LC-ATEA-SDTNYKATNFKEYL | Yes | |||
| HPV6 | LC-ASVT-TSSTYTNSDYKEYM | No | |||
| HPV16 | LC-AAISTSETTYKNTNFKEYL | No | |||
| HPV18 | ICASTQSPVPGQYDATKFKQYS | No | |||
| 13 | CD4 | E6 (111-130) | HPV1 | KLEVVSNGERVHRVRNRLKA | Yes |
| HPV2 | WEEKEALLVGNKRFHNISGR | Yes | |||
| HPV6 | LCEVEKVKHILTKARFIKLN | No | |||
| HPV16 | CPEEKQRHLDKKQRFHNIRG | No | |||
| HPV18 | PLNPAEKLRHLNEKRRFHNI | No | |||
| CD8 | L1 (166-180) | HPV1 | CTPASGEHW-TSRRCP | Yes | |
| HPV2 | CKPPIGEHWSKGTTCN | No | |||
| HPV6 | CAPPLGEHWGKGTQCT | Yes | |||
| HPV16 | CKPPIGEHWGKGSPCT | Yes | |||
| HPV18 | CAPAIGEHWAKGTACK | Yes | |||
| 15 | CD4 | L1 (176-90) | HPV1 | VFRVRFADPNRFAFG | Yes |
| HPV2 | VFHVKLPDPNKFGLP | Yes | |||
| HPV6 | VFKVVLPDPNKFALP | Yes | |||
| HPV16 | VFRIHLPDPNKFGFP | No | |||
| HPV18 | VFRVQLPDPNKFGLP | Yes | |||
a
The possibility that the ELISPOT assays may be picking up cross-reactive responses to other HPV types was investigated with cells from donors 12, 13, and 15. Samples were checked against peptides covering regions on HPV types 2, 6, 16, and 18 that corresponded to the HPV1 sequences recognized. These peptide sequences are shown in the fourth column of the table, and the last column indicates whether the peptide was recognized in each case.