FIG. 1.

Coprecipitation of HSV-1 proteins with Pol II holoenzyme. HEp-2 cells were infected with wt HSV-1 KOS or mock infected. When indicated, PAA (400 μg/ml) was added to infected cultures at the beginning of infection. Cells were harvested at 8 h p.i. Immunoprecipitation (IP) was performed on cell lysates using a rabbit polyclonal anti-hSRB7 antibody. Proteins in the cell lysates and immunoprecipitates were resolved by SDS-PAGE, and specific proteins were detected with monoclonal anti-Pol II antibody 8WG16 (1:1,000 dilution), rabbit anti-ICP8 antiserum 3-83 (1:1,000 dilution), monoclonal anti-ICP27 antibody H1113 (1:200 dilution), monoclonal anti-ICP0 antibody H1112 (1:200 dilution), anti-ICP4 monoclonal antibody 58S (1:200 dilution), and rabbit polyclonal anti-ICP22 antiserum R77 (1:500 dilution). (A) Coprecipitation of ICP27 and ICP8 with Pol II. (B) Lack of coprecipitation of ICP0, ICP4, and ICP22 with Pol II. The amount of cell lysate loaded on the gel relative to the amount of lysate used for immunoprecipitation was 1:20.