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. 2002 Jun;76(12):5893–5904. doi: 10.1128/JVI.76.12.5893-5904.2002

FIG. 2.

FIG. 2.

Coprecipitation of ICP8, ICP27, and Pol II holoenzyme with different Pol II antibodies. HEp-2 cells were infected with wt HSV-1 KOS or mock infected and harvested 8 h p.i. Immunoprecipitations were carried out on the cell lysate with a rabbit anti-hSRB7 antibody and the C21 rabbit anti-Pol II antibody. Proteins in cell lysates and immunoprecipitates were separated by SDS-PAGE and detected by Western blotting with anti-Pol II antibody 8WG16 (1:1,000 dilution), anti-ICP8 antibody 3-83 (1:1,000 dilution), and monoclonal anti-ICP27 antibody H1113 (1:200 dilution). The ratio of cell lysate loaded on the gel to the amount of lysate used in immunoprecipitation was 1:20. Lanes 1 and 2, cell lysates; lanes 3 and 4, anti-hSRB7 immunoprecipitates; lanes 5 and 6, C21 immunoprecipitates.