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. 2002 Jun;76(12):6311–6322. doi: 10.1128/JVI.76.12.6311-6322.2002

FIG. 3.

FIG. 3.

Dual RNA ISH and immunohistochemistry utilizing Vector red for FIV RNA probe detection, silver-enhanced immunogold for phenotypic identification, and methyl green counterstain. (A) p55+ FIV+ DC (arrows) in the vaginal submucosa (sm) of cat 3826 6 days p.i. Magnification, ×400. vm, vaginal mucosa. (B) FIV+ cell (arrow) and two FIV-uninfected MAC387+ macrophages (arrowheads) in the vaginal submucosa of cat 3826 6 days p.i. Magnification, ×200. (C) p55+ FIV+ cell (arrow) in the oral submucosa of cat 3804 10 days p.i. Magnification, ×200. oe, oral epithelium. (D) CD3+ FIV+ T cells (arrows) and several FIV-uninfected CD3+ cells (arrowheads) in the tonsillar parenchyma of cat 3795 10 days p.i. Magnification, ×400. (E) MAC387+ FIV+ macrophage (arrow) in a follicle (f) adjacent to the subcapsular sinus (scs) region of the retropharyngeal LN from cat 3815 3 days p.i. Magnification, ×400. (F) CD3+ FIV+ T cell (arrow) and several FIV-uninfected CD3+ T cells (arrowheads) in the PBMC of cat 3804 10 days p.i. Magnification, ×400.