FIG. 2.

JNK phosphorylation in MHV-infected cells. J774.1 cells were mock infected (Mk), MHV infected, or exposed to inoculum containing UV-irradiated MHV (UV). Cell lysates were harvested at the indicated times and analyzed by Western blot with a JNK phospho-specific antibody that detects JNK phosphorylated at residues Thr183 and Tyr185, shown in the upper panel (arrowheads), and a phosphorylation state-independent antibody that detects total JNK, shown in the lower panel (arrows). The upper arrow and upper arrowhead indicate JNK p54; the lower arrow and lower arrowhead indicate JNK p46.