TABLE 2.
Sequences and annealing positions within the HPV-31 genome of PCR primers used to make internal deletions of plasmids
| Primer no. | Primer name | Restriction site(s) | Genomic location(s) | Sequence (5′-3′)a |
|---|---|---|---|---|
| 14 | pCATPH13 (first half reverse) | EcoRV | 7020-7038 | AGAGATATCTGGTGTAGTGGTAGATGCT |
| 15 | pCATPH13 (second half forward) | EcoRV | 7142-7159 | TGTGATATCTGGTATATGTATGT |
| 16 | PCATPH14 (forward) | None | 7173-7186, 7211-7226 | GTGTACTTGTATAT/TATGCTATGTATGTTA |
| 17 | PCATPH14 (reverse) | None | 7173-7186, 7211-7226 | TAACATACATAGCATA/ATATACAAGTACAC |
| 18 | PCATPH15 (forward) | None | 7142-7161, 7211-7226 | ATATGGTATATGTATGTT/TATGCTATGTATGTTA |
| 19 | PCATPH15 (reverse) | None | 7142-7161, 7211-7226 | TAACATACATAGCATA/AACATACATATACCATAT |
| 20 | PCATPH16 (forward) | None | 7119-7131, 7211-7226 | GTATATGTGTGTG/TATGCTATGTATGTTA |
| 21 | PCATPH16 (reverse) | None | 7119-7131, 7211-7226 | TAACATACATAGCATA/CACACACATATAC |
| 22 | pCATPH17 (forward) | None | 7066-7081, 7212-7225 | ATGGATGTGTATGTAA/TATGCTATGTATGT |
| 23 | pCATPH17 (reverse) | None | 7066-7081, 7212-7225 | ACATACATAGCATA/TTACATACACATCCAT |
| 24 | MIE (reverse) | PstI, XhoI | 7198-7211 | GTCACTGCAGCTCGAGCATACACACATAAC |
a
Discontinuities in the annealing positions of primers are shown with a shill. Restriction sites are underlined.