TABLE 3.
Sequences and annealing positions within the HPV-31 genome (primers 25 to 32) and the HPV-16 genome (primers 33 to 36) of PCR primers used to make riboprobes
| Primer no. | Primer name | Promoter | Genomic location | Sequence (5′-3′)a |
|---|---|---|---|---|
| 25 | NLE (forward) | T7 (sense) | 7040-7062 | CGACTTAATACGACTCACTATAGCAAAACGTAAAAAAACTAAAAAG |
| 26 | 3′ NLE (forward) | T7 (sense) | 7085-7101 | TGTAATACGACTCACTATAGGGTGTGTCTGTATGTGTAT |
| 27 | NLE (reverse) | SP6 (antisense) | 7122-7140 | GATCGATTTAGGTGACACTATAGAACACACAAACACACACAT |
| 28 | 5′ NLE (reverse) | SP6 (antisense) | 7065-7084 | GATCGATTTAGGTGACACTATAGTGTATTACATACACATCCAT |
| 29 | M (forward) | T3 (sense) | 7161-7179 | CAGAGATGCAATTAACCCTCACTAAAGGGATATGTATGCGTGTGTACTT |
| 30 | M (reverse) | None | 7191-7210 | CATACACACATAACATACTA |
| 31 | S (forward) | T3 (sense) | 7283-7301 | CAGAGATGCAATTAACCCTCACTAAAGGGAGTAACATACTATTACTAT |
| 32 | S (reverse) | None | 7324-7343 | AGCAGGAACAAGTAGGAACA |
| 33 | NRE (forward) | T3 (sense) | 7128-7145 | CAGAGATGCAATTAACCCTCACTAAAGGGAGAATTCGCTAAACGCAAAAAACGT |
| 34 | 3′ NRE (forward) | T3 (sense) | 7177-7192 | CAGAGATGCAATTAACCCTCACTAAAGGGAGAATTCGTGTTGTTTGTTGTGT |
| 35 | 5′ NRE (reverse) | None | 7155-7176 | TAGAGCTCTAATTCAACATACATACAATAC |
| 36 | NRE (reverse) | None | 7186-7207 | CTGAGCTCACATACAAACATATACACAAC |
a
Bacteriophage RNA polymerase promoter sequences are underlined.