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oriS is required in both amplicon plasmids for efficient IM-HR
| Amplicon plasmids | No. of blue coloniesa
|
% IM-HR | |
|---|---|---|---|
| Ampr | Kanr | ||
| pW7 + pW2-lacZ | 324 | 741 | 43.7 |
| pW7-del + pW2-lacZ | 2 | 700 | 0.3 |
| pW7 + pW2-lacZ-del | 8 | 112 | 7.1 |
| pW7-del + pW2-lacZ-del | 0 | 200 | 0 |
Values are averages for two repeated experiments.
