Figure 5. Functional characterization of MakA, MakB, and MakE using liposome assays.

a) Liposome co-sedimentation assay to assess the membrane association of MakA, MakB, MakE, and the MakA/MakB/MakE mixture. Liposome-bound proteins are present in the pellet fraction. The right panel shows a negative control without liposomes. b) Liposome co-floatation assay to assess the membrane association of MakA, MakB, MakE, and the MakA/MakB/MakE mixture. Liposomes float to the top layer and their associated proteins are examined by SDS-PAGE. c) Liposome leakage induced by MakA, MakB, MakE, or the MakA/MakB/MakE mixture. Leakage is shown as a percentage of the maximum fluorescence signal as a function of time. Maximum fluorescence intensity was measured upon full SRB molecules release after β-OG-mediated liposome disruption. d) Quantification of the leakage at 1500 s. Bars represent average of the normalized fluorescence intensities performed in triplicates. Error bars represent standard deviations.