Table 1.
Cryo-EM data collection and atomic model building
| Data collection | |
|---|---|
| Sample name | MakA |
| Instrument | Titan Krios G2 |
| Detector | K3 Summit |
| Energy filter | BioQuantum |
| Objective aperture | No |
| Nominal magnification | 105,000× |
| Data collection mode | Beam-Image Shift; 3×3 holes, 1 shot/hole |
| Electron dose rate (e − /pixel/s) | 12.5 |
| Exposure time (s) | 5 |
| Detector pixel size (Å) | 0.833 |
| Data pixel size (Å) | 0.4165 |
| Movies acquired | 2,234 |
| Reconstruction | |
| Molecular weight (kDa) | 39 × 6 = 234 |
| Reconstruction symmetry | C3 |
| Particles used in reconstruction | 236,459 |
| Resolution FSC0.143 (Å) | 3.13 (global)/2.68 (local) |
| Atomic model building | |
| Non-hydrogen atoms | 15,201 |
| Protein residues | 2,052 |
| Ligands | 0 |
| RMSD bond lengths (Å) | 0.014 |
| RMSD bond angles (°) | 1.035 |
| Model-to-map CC (mask) | 0.88 |
| MolProbity score | 1.00 |
| Clashscore (all atom) | 2.25 |
| Poor rotamers (%) | 0 |
| Ramachandran (%) | |
| favored | 98.48 |
| allowed | 1.52 |
| outliers | 0 |