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. 2002 Jun;76(12):6332–6343. doi: 10.1128/JVI.76.12.6332-6343.2002

FIG. 6.

FIG. 6.

The effects of cell surface GAGs on HIV-1 infectivity are independent of CypA incorporation. (A) The infectivity of wild-type or capsid mutant derivatives (G89V and P90A/A92E) of NL/HXB and NL/ADA was measured by using CHO-K1- and pgsA745-derived target cells. The infectious titer of each virus on each cell line was measured as described for Fig. 2 with normalization for minor variation in the amount of p24 in each viral supernatant. (B) NL/HXB and NL/ADA virus stocks were produced in 293T cells in the presence or absence of CsA, and infectious titers were measured after normalization, as for panel A, by using CHO-K1- and pgsA745-derived target cells. (C) NL/HXB and NL/ADA were each pseudotyped with the VSV-G envelope protein and introduced into the unmodified Jurkat cell line or a derivative lacking both copies of the cypA gene. Progeny virions were harvested, and the infectivity of each was measured with CHO-K1 and pgsA745 target cells. For each experiment, the results presented are the means and standard deviations of three titrations.