Immunogold detection of structural proteins expressed in UCp7con-9.1 cells. (a) E1 protein is found on the budding membrane invaginations in the lumen of the rER. (b) Core protein is also found on membranes in the lumen of the rER and, in addition, on lipid droplets (arrowheads). (c) Double immunogold staining shows proteins E1 (larger gold grains) and core (smaller gold grains) on the same membrane structures, albeit not closely associated with each other. L, lumen of rER; N, nucleus. Bars, 0.5 μm.