TABLE 1.
Genomic loci of pREMIh integration associated with enhanced 3×FLAG secretion.
| K. phaffii gene | Locus Tag | BLASTp vs. S. cerevisiae | Putative function | REMI modification |
|---|---|---|---|---|
| AQR1 | BQ9382_C2-0360 | 8e−100/96% | Plasma membrane transporter | Disrupted ORF |
| FMS1-2 | BQ9382_C4-0690 | 2e−44/97% | Polyamine oxidase | Disrupted ORF |
| SKG3 | BQ9382_C4-1750 | 5e−140/47% | Unknown; bud neck-localized | Disrupted ORF |
| CDC48 | BQ9382_C4-0255 | 0.0/100% | AAA ATPase, protein unfoldase | C-terminal truncation |
| NUP120 | BQ9382_C4-5030 | 6e−08/16% | Nuclear pore complex | Disrupted ORF |
| YDL206W | BQ9382_C3-5285 | 4e−32/87% | Cation transporter | 5′UTR insertion |
| PHS1 | BQ9382_C3-5291 | 9e−57/93% | 3-Hydroxyacyl-CoA dehydratase | 5′UTR insertion |
| DCP1 | BQ9382_C1-0840 | 9e−28/95% | mRNA decapping | 5′UTR insertion |
| SLC1 | BQ9382_C1-0835 | 1e−90/85% | Acylation of lysophosphatidic acid | 5′UTR insertion |
| MAK11 | BQ9382_C1-3975 | 4e−108/90% | 60S ribosomal subunit biogenesis | C-terminal truncation |
BLASTp e-values and query coverage are given for the best S. cerevisiae homolog. Putative gene functions were inferred from similarity. Assigned functions/annotations for SKG3 and NUP120 are tentative due to low BLASTp, query coverage.