FIG. 1.

DC-SIGN expression on myeloid lineage cells. (A) DC stained with MAbs against DC-SIGN or L-SIGN. DC were first incubated with each of the MAbs, after which FITC-conjugated anti-mouse IgG and PE-conjugated anti-human CD11b were used for double staining. PE-conjugated mouse IgG2a and FITC-conjugated mouse IgG1, IgG2a, and IgG2b were used as isotype control antibodies (top row). (B) DC-SIGN expression of cytokine-treated monocytes. As depicted to the left of the FACS panel sets, purified CD14⁺ monocytes were cultured for 4 days in the presence of no cytokines, IL-2 (200 U/ml), IL-4 (100 ng/ml), IL-6 (1,000 U/ml), IL-12 (20 ng/ml), IL-13 (100 ng/ml), or GM-CSF (50 ng/ml), singly or in combination. Cells were stained with DC-SIGN MAb 507(D) or isotype control antibody (IgG2b), followed by PE-conjugated goat anti-mouse Ig antibody, or stained with PE-conjugated CD14 antibody. Antibody staining (FL2) is depicted by the histogram plots along the x axis.