FIG. 3.

HIV-1 transmission mediated by THP-1/DC-SIGN cells is blocked with the DC-SIGN-specific MAbs. HIV-1 capture and transmission of R5-tropic HIV-Luc/JRFL (A) and X4-tropic HIV-Luc/HXB2 (B) was tested. THP-1 or THP-1/DC-SIGN donor cells were incubated with each of the MAbs (10 μg/ml) for 30 min at 37°C, and then HIV-luciferase pseudotyped virus was incubated with the cells for 3 h at 37°C. The cells were washed and cocultured with Hut/CCR5 target cells in the presence of Polybrene. HIV-1 infection was determined after 2 days by measuring the luciferase activity. No Ab, treatment without antibody. Mouse IgG was used as a nonspecific antibody control. Each data set represents the mean of three or four separate wells of infected cells. cps, counts per second.