FIG. 3.

MDDC bind Ebola virus GP-pseudotyped particles and transmit infectivity to susceptible cells. MDDC were incubated with infectious supernatants containing VSV-G-, Ebola virus Zaire (Ebo-Z)-, or Ebola virus Reston (Ebo-R) GP-pseudotyped lentiviruses after a brief preincubation in the absence or presence of MR-1 DC-SIGN-specific monoclonal antibody. Cells were extensively washed thereafter and plated onto HeLa cells. The same amount of infectious supernatant (Sup) without incubation with DC was directly added to the HeLa cells as a control of the original infectivity. Cells were assayed for luciferase 48 h after infection. The experiment was performed with cells from two independent donors, and a representative result is shown.