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. 2002 Jul;76(13):6442–6452. doi: 10.1128/JVI.76.13.6442-6452.2002

FIG. 5.

FIG. 5.

Identification of mouse ASCT proteins on the surfaces of CHO cells. CHO cells stably expressing myc-tagged mASCT1, mASCT2, and their N-deglycosylated mutant proteins were surface biotinylated as described in Materials and Methods. Samples of the total protein cell lysates were also used in a parallel analysis. The biotinylated proteins were then purified by affinity chromatography. The samples were analyzed by Western immunoblotting with anti-myc tag monoclonal antibody 9E10 (Sigma). (A) Total cellular myc-tagged mASCT1, mASCT2, and their N-deglycosylated mutants proteins; (B) cell surface myc-tagged mASCT1, mASCT2, and their N-deglycosylated mutants proteins that were biotinylated on cell surfaces and then affinity purified prior to immunoblot analysis. Numbers on the left are molecular weights in thousands.