TABLE 1.
Spinoculation and deletion of the cytoplasmic tail of HERV-W enhance HIV/HERV-W pseudotype titersa
| Treatment | Target cell | Titer (CFU/ml)b of the indicated virus pseudotypes
|
|||
|---|---|---|---|---|---|
| HIV-LacZ
|
HIV-GFP
|
||||
| HERV-W | HERV-Wcyt16 | HERV-W | HERV-Wcyt16 | ||
| No spinoculation | TE671 | 1.2 × 103 | 2 × 104 | 1.6 × 103 | 5.2 × 104 |
| CHO | 10 | 22 | 16 | 12 | |
| Spinoculation | TE671 | 7.8 × 104 | 1 × 105 | 4.4 × 104 | 1.6 × 105 |
| CHO | 22 | 12 | 40 | 12 | |
a
Human TE671 cells were tested for susceptibilities to HIV-LacZ(HERV-W), HIV-LacZ(HERV-Wcyt16), HIV-GFP(HERV-W), and HIV-GFP(HERV-Wcyt16) pseudotype viruses. Hamster CHO cells served as negative control for all of these viruses.
b
The titers are averages from two independent infection studies