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Differential requirements of peptide concentration for maximal CTL function against the three CTL epitopes. Three different concentrations (0.04, 0.4, and 4 μM) of the minimal epitope peptides were used to sensitize target cells (EL-4Ks) for lysis by CNS-infiltrating CTL. A representative result of three separate experiments is shown here. The spontaneous release was less than 14%.
