FIG. 8.

Binding of NS1 to purified RPA. Purified RPA complex comprising three (p70, p34, p14) or two (p70, p34) subunits or E. coli SSB was immobilized on ELISA wells and incubated with increasing amounts of purified GST-NS1. The different target proteins are indicated at the right. In each ELISA, bound NS1 was detected by a rabbit polyclonal antiserum directed against its carboxyl terminus, followed by horseradish peroxidase-coupled anti-rabbit immunoglobulin G antibody and a chromogenic substrate. ELISA wells coated with BSA served as negative controls. Inclusion of DNase I or micrococcal nuclease in these assays did not quantitatively or qualitatively affect the observed interactions. O.D., optical density.