FIG. 2.

US8 does not affect transport of MHC class I products. U373 or U373+US8 cells were labeled with [³⁵S]methionine-cysteine for 5 min and chased with nonradioactive medium for the indicated time periods prior to lysis in 0.5% NP-40. Immunoprecipitations were performed with either monoclonal antiserum W6/32, which recognizes folded MHC class I molecules (A), or a monoclonal antiserum that recognizes the transferrin receptor (B). The samples were digested with EndoHf prior to analysis by SDS-PAGE.