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Subcellular distribution of the UL48 protein. RK13 cells were fixed 20 h after infection with PrV-Ka with a 1:1 mixture of methanol and acetone and incubated with UL48-specific rabbit serum (α-UL48). Separate and merged fluorescence of Alexa 488-conjugated anti-rabbit antibodies (green) and of propidium iodide-stained chromatin (red) was analyzed in a confocal laser scan microscope. Bar, 50 μm.
