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Induction of immediate-early gene expression by the UL48 protein of PrV. RK-UL48 (lanes a) and normal RK13 cells (lanes b) were infected with either PrV-Ka or PrV-ΔUL48 and incubated in the presence of cycloheximide for 3 or 6 h. Total RNA of infected and noninfected (n.i.) cells was separated in a denaturing agarose gel (left) and transferred to a nylon membrane. The blot was hybridized with a probe specific for the major immediate-early transcript of PrV (IE180 mRNA; right).
