TABLE 5.
Fusion inhibition with human monoclonal antibodies specific for gp41 conformational epitopes
| Protocola | Antibodyb (dose, μg/ml) | Specificity | % Fusion inhibition at 37°C (ID50, μg/ml) |
|---|---|---|---|
| A | MAb 240-D (30) | Cluster I | 0 |
| MAb 181-D (30) | Cluster I | 0 | |
| MAb 98-6 (30) | α-C-HR | 18 | |
| MAb 1281 (30) | α-Bundle | 0 | |
| MAb 167-D (30) | α-Bundle | 0 | |
| MAb 2F5 (30) | ELDKW | 78 (2.5) | |
| B | MAb 240-D (30) | Cluster I | 0 |
| MAb 181-D (30) | Cluster I | 1 | |
| MAb 98-6 (30) | α-C-HR | 75 (15) | |
| MAb 1281 (30) | α-Bundle | 52 (25) | |
| MAb 167-D (30) | α-Bundle | 64 (10) | |
| MAb 2F5 (30) | ELDKW | 65 (4.2) | |
| C | MAb 240-D (30) | Cluster I | 0 |
| MAb 181-D (30) | Cluster I | 0 | |
| MAb 98-6 (30) | α-C-HR | 38 (90) | |
| MAb 1281 (30) | α-Bundle | 78 (4) | |
| MAb 167-D (30) | α-Bundle | 74 (3.5) | |
| MAb 2F5 (30) | ELDKW | 60 (6.2) |
a
Protocol A, E + Ab (37°C, 1 h) → add T cells; protocol B, E/T + Ab (31.5°C, 1 h) → 37°C; protocol C, E/T (31.5°C, 1 h) → add Ab (31.5°C, 1 h) → 37°C.
b
MAb, monoclonal antibody.