FIG. 2.

Time course of HIV-1 penetration across the BBB model. One million HIV-1_JR-FL RNA copies were placed in the upper chambers of replicate BBB models, and HIV-1 penetration into the lower chambers was measured by a HIV-1 RNA (Amplicor) assay. On the vertical axis are shown the titers in the lower chamber (mean ± standard error of the mean) at the indicated times (the titer at 1 h p.i. was below the limit of detection [<300 RNA copies]). The infectious assay showed that at 48 h p.i., the titers were >10⁻³ TCID₅₀ in the upper chamber and 10^−1.3 TCID₅₀ in the lower chamber. The experiment was repeated three times with comparable results.