FIG. 2.

Detection of c-fms, CSF-1, and c-myc transcripts. Total RNA was isolated from both cells. RNA was reverse transcribed and amplified with pairs of PCR primers specific for the c-fms (A) or CSF-1 (B) gene. Specific products were visualized by ethidium bromide staining on a 1.8% agarose gel. (C) Northern blot experiments performed with total RNA from the cell lines listed and hybridized with c-myc- and GAPDH-specific probes. MW, molecular weight markers.