TABLE 1.
Transcript | Oligomer | Fragment length (bp) | Sequence |
---|---|---|---|
LMP-1 | 5′ primerb | 408 | CAC GAC CTT GAG AGG GGC CCA |
3′ primerb | GCC AGA TGG TGG CAC CAA GTC | ||
5′ primerc | 399 | CCT TTG CTC TCA TGC TTA TAA | |
3′ primerc | GCC AGA TGG TGG CAC CAA GTC | ||
AS2d | CTC TCA AGG TCG TGT TCC AT | ||
Sc2 (scrambled)d | TTC GAC TAG ACT CCG GTT TC | ||
EBNA-1 Qp/Cp | 5′ primerb | 421 | GTA ACT TAG GAA GCG TTT CT |
3′ primerb | GGT CTC CGG ACA CCA TCT CT | ||
5′ primerc | 80 | GGA CCT CAA AGA AGA GGG GG | |
3′ primerc | GCT CCT GGT CTT CCG CCT CC | ||
EBNA-2 | 5′ primere | 177 | CTA TCT TGC GTT ACA TGG GGG ACA |
3′ primere | GGT CTC CGG ACA CCA TCT CT | ||
BZLF-1 | 5′ primer | 430 | CAC CTC AAC CTG GAG ACA AT |
3′ primer | TGA AGC AGG CGT GGT TTC AA | ||
Probe | GCA CAT CTG CTT CAA CAG GA | ||
c-fms | 5′ primer | 293 | CTT TCC TAA TCC CCT TAT C |
3′ primer | ATT ACA GCA GTA CCA GTA TG | ||
CSF-1 | 5′ primer | 192 | TCG GAC GCA GGC CTT GTC ATG |
3′ primer | GAA CAG TTG AAA GAT CCA GTG |
Oligonucleotides used in experiments involving PCR and sequencing are shown in Table 2.
For RT-PCR.
For PCR.
For antisense strategy.
For PCR and RT-PCR.