TABLE 3.
Cell surface phenotype of monocytic cell lines E1 and TE1
| Marker | Resulta for:
|
Clone | Isotype | Supplier | Fluorochrome | |
|---|---|---|---|---|---|---|
| E1 | TE1 | |||||
| CD3 | − | − | UCHT1 | IgG1 | Immunotech | FITC |
| CD2 | − | − | 39C1.5 | IgG2a | Immunotech | FITC |
| CD4 | +w | +w | 13B8.2 | IgG1 | Immunotech | FITC |
| CD8 | − | − | B9.11 | IgG1 | Immunotech | FITC |
| CD25 | − | − | ACT-1 | IgG1 | Dako | FITC |
| CD28 | − | − | CD28.2 | IgG1 | Immunotech | FITC |
| HLA-DR | + | + | B8.12.2 | IgG2b | Immunotech | FITC |
| κ chain | − | − | Polyclonal | Polyclonal | Dako | FITC |
| λ chain | − | − | Polyclonal | Polyclonal | Dako | PE |
| CD19 | − | − | J4.119 | IgG1 | Immunotech | PE |
| CD20 | − | +w | B9E9(HRC20) | IgG2a | Immunotech | FITC |
| CD10 | − | − | ALB2 | IgG2a | Immunotech | FITC |
| CD21 | +w | +w | BL13 | IgG1 | Immunotech | FITC |
| CD22 | − | − | 4KB128 | IgG1 | Dako | FITC |
| CD14 | + | + | RMO52 | IgG2a | Immunotech | FITC |
| CD80 | + | + | MAB104 | IgG1 | Immunotech | None |
| CD86 | − | − | 2331(FUN-1) | IgG1 | Pharmingen | None |
| CD1a | − | − | BL6 | IgG1 | Immunotech | None |
| CD16 | − | − | 3G8 | IgG1 | Immunotech | FITC |
| CD34 | − | − | 8G12 | IgG1 | Becton Dickinson | PE |
| CD54 | + | + | 84H10 | IgG1 | Immunotech | PE |
a
−, negative; +, positive; +w, weakly positive. Comparisons are to results for the control antibody.