TABLE 5.
Production of three monokines (TNF-α, IL-6, and IL-8) in cell supernatants after 40 h of culture with (activated) or without (constitutive) cell activation by PMA and ionomycin
| Cell line | Expression | Levela of:
|
||
|---|---|---|---|---|
| TNF-α (U/ml) | IL-6 (pg/ml) | IL-8 (pg/ml) | ||
| Jurkat | Constitutive | < | < | 15 |
| Activated | < | < | 497 | |
| E1 | Constitutive | < | 10.2 | 356 |
| Activated | 14 | 15.4 | 20,520 | |
| TE1 | Constitutive | < | < | 780 |
| Activated | 26 | 18.4 | 24,740 | |
| U937 | Constitutive | < | 10.3 | 75 |
| Activated | < | 36.4 | 5,098 | |
| HL60 | Constitutive | < | < | 261 |
| Activated | < | < | 8,053 | |
a
IL-6 and IL-8 levels were evaluated by enzyme immunoassay, and TNF-α levels were determined by a cytoxicity test against L929.<, lower than the sensitivity threshold of the test.