FIG. 1.
Neutralization of OSU infectivity by MAbs directed at VP7 (1C10) and VP8∗ (4E8 and 5G7). Purified OSU virions (100 μl; 20 ng/ml; 2 × 105 FFU/ml) were mixed with purified MAbs (100 μl) at different concentrations (0.06 to 60 μg/ml) and incubated at 37°C for 1.5 h. Virus-antibody mixtures were serially diluted and inoculated in duplicate onto MA-104 cells grown in 96-well plates. After further incubation at 37°C for 1.5 h, the inoculum was removed and fresh MEM was added to the cells. The next day (12 to 18 h p.i.), the cells were fixed with methanol and immunostained for FFU. Results are expressed as percent residual infectivity after neutralization compared with the infectivity of the virus incubated without antibodies. Results from one experiment are shown. The experiment was repeated twice with similar results.