FIG. 2.

Kinetics of decapsidation of purified OSU (left) and RRV (right) virions at different Ca²⁺ concentrations, as analyzed by perpendicular light scattering. Ten microliters of purified OSU (200 μg/ml) or RRV (40 μg/ml) virions was added to a stirred cuvette containing 1.1 ml of EGTA-Ca-EGTA buffers adjusted to various Ca²⁺ concentrations (as indicated) and maintained at 25°C (see Materials and Methods). Results are presented as relative scattering as described in Materials and Methods. Results of one experiment from a series of two are shown.