FIG. 4.

Kinetics of decapsidation of purified OSU virions reacted with different concentrations of purified MAb 1C10 directed against VP7. Purified OSU (10 μl; 200 μg/ml) virions were mixed with different concentrations of MAb 1C10 (2 μl) and incubated for 1 h at room temperature. Virus-antibody mixtures (12 μl) were added to a stirred cuvette containing 1.1 ml of EGTA-Ca-EGTA buffers adjusted to a 150 nM Ca²⁺ concentration and maintained at 25°C. MAb concentrations in the mixture are indicated. Results of one experiment from a series of two are shown.