FIG. 6.

Kinetics of neutralization and aggregation of OSU virus. Purified OSU (3 μg/ml) virions were mixed with MAb 1C10 at different concentrations and incubated for 1 h at room temperature. (A) Aliquots of each virus-antibody mixture were observed directly by EM. Each mixture was identified by a number from 1 to 5. Bar, 100 nm. (B) The same virus-antibody mixtures for which images are shown in panel A were diluted, inoculated onto MA-104 cell monolayers in 96-well plates, and assayed for residual infectivity. Results are expressed as percent residual infectivity after neutralization compared with the infectivity of the virus incubated without antibodies. (C) Residual infectivity after neutralization (data from Fig. 6B) and inhibition of decapsidation after incubation with different MAb 1C10 concentrations (data from Fig. 4) were plotted as functions of the MAb/virus ratio.