Table 2.

The repeatability tests of the multiplex qPCR.

Panel	Templates	Concentrations (copies/μL)	Inter-assay		Intra-assay
			Cq values (mean ± SD)	CV%	Cq values (mean ± SD)	CV%
I	M. ovipneumoniae	104	28.35 ± 0.42	2.12	28.15 ± 0.47	1.67
		105	23.74 ± 0.58	2.45	24.50 ± 0.28	1.14
		106	19.41 ± 0.41	1.48	19.87 ± 0.11	0.55
	M. mycoides subsp. capri	104	30.64 ± 0.31	1.02	30.05 ± 0.83	2.76
		105	26.47 ± 0.82	3.10	27.23 ± 0.71	2.61
		106	20.11 ± 0.17	0.85	21.60 ± 0.21	0.97
	M. capricolum subsp. capripneumoniae	104	27.63 ± 0.64	2.32	28.77 ± 0.79	2.75
		105	23.42 ± 0.09	0.38	25.10 ± 0.49	1.95
		106	19.90 ± 0.12	0.60	20.75 ± 0.16	0.77
II	P. multocida	104	28.92 ± 0.44	1.52	31.02 ± 0.85	2.74
		105	25.80 ± 0.67	2.60	24.18 ± 0.24	0.99
		106	22.45 ± 0.31	1.38	22.25 ± 0.27	1.21
	M. haemolytica	104	27.33 ± 0.82	3.00	25.88 ± 0.62	2.39
		105	24.67 ± 0.22	0.89	22.71 ± 0.36	1.59
		106	20.10 ± 0.15	0.75	20.34 ± 0.14	0.69
	S. equi subsp. zooepidemicus	104	29.40 ± 0.76	2.59	29.30 ± 0.68	2.32
		105	26.18 ± 0.53	2.02	26.44 ± 0.55	2.08
		106	21.05 ± 0.19	0.90	23.92 ± 0.33	1.38