Cell lysis buffer:	40 mM Tris-HCl (pH 7.5), 0.5 mM tris(2-carboxyethyl)phosphine (TCEP), 10% (v/v) glycerol, 0.3 M NaCl
R buffer:	20 mM Tris-HCl (pH 7.5), 0.5 mM TCEP, 10% (v/v) glycerol
R1 buffer:	20 mM Tris-HCl (pH 7.5), 0.5 mM TCEP, 10% (v/v) glycerol, 0.4 M NaCl, 0.005% (w/v) Tween 20
R2 buffer:	20 mM Tris-HCl (pH 7.5), 0.5 mM TCEP, 10% (v/v) glycerol, 0.4 M NaCl
R3 buffer:	20 mM Tris-HCl (pH 7.5), 0.5 mM TCEP, 10% (v/v) glycerol, 0.4 M NaCl and 0.01% (w/v) Tween 20
R4 buffer:	20 mM Tris-HCl (pH 7.5), 0.5 mM TCEP, 10% (v/v) glycerol, 0.3 M NaCl, 0.005% (w/v) Tween 20