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. 2026 Sep 1;17:10398. doi: 10.1038/s41467-026-77122-x
ATPase reaction buffer: 25 mM HEPES-KOH (pH 7.5), 1 mM TCEP, 5% (v/v) glycerol, 75 mM potassium acetate (KOAc), 30 mM KCl, 1 mM magnesium acetate (Mg(OAc)2), 0.01% (w/v) NP-40
SDB buffer 20 mM Tris-HCl (pH 7.5), 1 mM TCEP, 15% glycerol, 100 mM KCl, 1 mM Mg(OAc)2, 0.05% (w/v) NP-40
SL buffer: 25 mM HEPES-KOH (pH 7.5), 1 mM TCEP, 5% (v/v) glycerol, 75 mM KOAc, 30 mM KCl, 1 mM Mg(OAc)2, 0.05% (w/v) NP-40
IP500 buffer: 25 mM HEPES-KOH (pH 7.5), 500 mM NaCl, 0.25% (w/v) NP-40, 1 mM ethylenediaminetetraacetic acid (EDTA)
IP100 buffer: 25 mM HEPES-KOH (pH 7.5), 100 mM NaCl and 0.1% (w/v) NP-40
Elution buffer: 10 mM Tris-HCl (pH 7.5), 0.5% (w/v) sodium dodecyl sulfate (SDS), 1 mM EDTA, 1 mg mL–1 proteinase K
RE buffer: 25 mM Tris-HCl (pH 7.5), 1 mM TCEP, 10 mM MgCl2, 100 mM NaCl and 0.1% (w/v) NP-40
DC500 buffer: 25 mM HEPES-KOH (pH 7.5), 500 mM NaCl, 1 mM EDTA, 0.05% (w/v) NP-40
DC100 buffer: 25 mM HEPES-KOH (pH 7.5), 100 mM NaCl, 0.05% (w/v) NP-40
AFM-A buffer: 25 mM HEPES-KOH (pH 7.5), 1 mM TCEP, 10% (v/v) glycerol, 300 mM KOAc, 1 mM Mg(OAc)2
AFM-B buffer: 25 mM HEPES-KOH (pH 7.5), 1 mM TCEP, 5% (v/v) glycerol, 75 mM KOAc, 1 mM Mg(OAc)2
AFM-C buffer: 25 mM HEPES-KOH (pH 7.5), 1 mM TCEP, 10% (v/v) glycerol, 30 mM KOAc, 1 mM Mg(OAc)2