FIG. 4.

Effect of the dose of IRF DNA on raised Ab. (A) For i.m. immunizations, mice were injected with 10 μg of HA-expressing DNA immunogen (pJW4303/H1) and the indicated doses (10 or 100 μg) of an IRF-expressing DNA (pGA/IRF). The low-dose groups were supplemented with 90 μg of mock DNA to adjust the total amount of DNA to 110 μg. (B) For g.g. immunization, gold beads delivered 0.1 μg of pJW4303/H1 and the indicated dose (0.1 or 1.0 μg) of pGA/IRF and the low-dose groups were adjusted with 0.9 μg of mock DNA to a total of 1.1 μg of DNA per shot. Data are expressed as the geometric means of 4 to 5 individual mice ± standard deviations (error bars). The immunization schedule was the same as depicted in Fig. 3A.