TABLE 1.
NNRTI susceptibilities of HIV-1 variants constructed by site-directed mutagenesisa
| Mutation(s) | Fold increase in IC50 of:
|
||
|---|---|---|---|
| DLV | EFV | NVP | |
| K103N | 56 | 15 | >60 |
| Y181C | 36 | 1.1 | >60 |
| Y318F | 42 | 1.1 | 3.0 |
| K103N/Y181C | >100 | 23 | >60 |
| K103N/Y318F | >100 | 43 | >60 |
| Y181C/Y318F | >100 | 3.3 | >60 |
| K103N/Y181C/Y318F | >100 | 84 | >60 |
a
Mutations were introduced in HIV-1 RT by site-directed mutagenesis, and the mutant RT was transferred into the HXB2-D wild-type background. The susceptibilities to the three NNRTIs were assessed as described in the text. The data represent the geometric mean for two independent assays. In some cases (indicated by “>”) observed fold changes exceeded the maximum that could be assessed in the assay.