FIG. 3.

Verification of sensitivity of the assay used for determination of relative genotypic ratios of viruses in mixed stocks. Shown are sequencing electropherograms of the 5′ +1 HRE half-site region of PCR products generated from viral DNA recovered from various mixtures of stocks of WT and +1/+55 HRE DMT virus. Percentages of WT virus present in the mixture are given on the left. Vertical rectangles, bases within the electropherograms that differ between the mutant and wild type; horizontal rectangles (bottom), half-site sequences. Black peak, wild-type guanosine base; blue peak, mutant cytidine base.