FIG. 5.

Wild-type virus has no replication advantage over the double HRE mutant virus in CV-1PD′ cells. Shown are sequence electropherograms of the +1 HRE 5′ half-site and the +55 HRE 3′ half-site regions of PCR products generated from viral DNA recovered from the initial virus stock (starting) used for infection of CV-1PD′ cells with WT and +1/+55 HRE DMT in an initially equal, low-MOI coinfection and from the first and second harvests following sequential infection and incubation at 37°C for 10 days in DMEM containing 2% FBS. Virus competition experiments were performed in triplicate. The electropherograms are representative of the data obtained from one set of experiments. For the significance of colors and of the vertical and horizontal rectangles, see the Fig. 3 legend.