FIG. 4.

Apical-basolateral cell surface expression of PRV gB. Polarized monolayers of ST cells, grown on permeable filter supports, were inoculated with PRV Be (A) or PRV Y902A (B) at an MOI of 10. At 8 h p.i., cells were washed, paraformaldehyde fixed, washed again, and incubated subsequently from both the apical and basolateral sides with mouse anti-gB antibodies and FITC-labeled goat anti-mouse antibodies. Nuclei were counterstained using propidium iodide. Vertical sections through the z axis of the cells are shown with viral glycoproteins in panels 1 and nuclei in panels 2. The arrow indicates a PRV Y902A-infected cell with apical expression of gB. Bar, 10 μm.