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. 2026 Sep 13;11(38):57830–57844. doi: 10.1021/acsomega.6c07048

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UV–vis absorption spectra of NPs functionalized with FITC. (a) Absorption spectrum of AgNPs-cit-l-cys (black) and AgNPs-cit-l-cys-FITC (red). Absorption peaks have a maximum around 400 nm. (b) Absorption spectrum of AgNPs-PEG (black) and AgNPs-PEG-FITC (red). Absorption peaks have a maximum around 400 nm, and there is evident a slight shoulder around 500 nm. (c) Absorption spectrum of AuNPs-cit-l-cys (black) and AuNPs-cit-l-cys-FITC (red) showing an increase in absorption in the 490–500 nm region, indicative of FITC. (d) Absorption spectrum of AuNPs-PEG (black) and AuNPs-PEG-FITC (red). Absorption peaks have a maximum around 550 nm. e-f) Fluorescence emission spectrum of FITC recorded in solution of functionalized NPs. e) represents the emission spectrum of AuNPs-cit-l-cys-FITC; f) emission spectrum of AgNPs-cit-l-cys-FITC. g) Emission spectrum of AuNPs-PEG-FITC. h) Emission spectrum of AgNPs-PEG-FITC. The emission maximum is observed around 515 nm, a wavelength characteristic of FITC.