Skip to main content
. 2026 Sep 13;11(38):57830–57844. doi: 10.1021/acsomega.6c07048

5.

5

Flow cytometry investigations reported the fluorescence signal detected on the FITC channel for: a) AuNPs-PEG. The red peaks represent the negative signal corresponding to nanoparticles before functionalization with FITC (MFI:1698; Freq: 10,4%). The blue signals, composed of both negative and positive populations, correspond to FITC-functionalized NPs (MFI: 11079; Freq: 54,6%). b) AuNPs-cit-l-cys. The red peaks represent the negative signal corresponding to nanoparticles before FITC grafting (MFI:364; Freq: 1.82%). The blue signals, composed of both negative and positive populations, correspond to FITC-functionalized NPs (MFI: 15395; Freq: 45.8%). c) Comparison of the FITC signal MFI between FITC-functionalized AuNPs-PEG-FITC (blue) and AuNPs-cit-l-cys-FITC (light blue). The blue peak shows a weak positive signal with an MFI of 8973. In contrast, the light blue peak, clearly visible in the positive region, has a higher MFI of 21330. These results indicate a greater efficiency of FITC functionalization for AuNPs-cit-l-cys.