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. 2026 Jul 8;28:166. doi: 10.1186/s13058-026-02334-4

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© The Author(s) 2026

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Correlation between HER2 protein expression (H-score) and ERBB2 mRNA levels in HER2-negative breast cancer. Scatterplot illustrating the relationship between HER2 immunohistochemistry H-score and ΔCt values for ERBB2 mRNA expression (relative to RPL0). The orange line represents the linear regression fit, with shaded area indicating the 95% confidence interval