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. Author manuscript; available in PMC: 2026 Oct 2.

Published in final edited form as: Transplant Cell Ther. 2026 Feb 2;32(6):670.e1–670.e14. doi: 10.1016/j.jtct.2026.01.031

Figure 7. — Transcriptomic profiling of donor-derived hematopoietic cells following transplantation. Bulk RNA sequencing was performed on H-2K^b donor-derived Lin- cells isolated from BALB/c recipients of WT or NOD1×2^−/− HSPCs 6 wk after stem cell transplantation (n = 3 mice/group). (Panel A) Volcano plot of differentially expressed genes comparing WT versus NOD1/2^−/−donor-derived cells, with upregulated genes shown in red and downregulated in blue. (Panel B, left) Principal component analysis (PCA) demonstrating separation of WT and NOD1/2^−/− chimera samples; (right) correlation heatmap confirming within-group clustering. (Panel C-D) Pathway enrichment analysis of differentially expressed genes. Bubble size (C) corresponds to pathway magnitude. The x-axis represents statistical significance (−log₁₀ pORA), and the y-axis shows pathway impact (−log₁₀ pAcc). The summary table (D) depicts the most significantly enriched pathways among differentially expressed genes based on over-representation and impact analyses. The table p-values, false discovery rate (FDR), and Bonferroni-adjusted p-values confirm the high statistical significance of these pathways. (Panels E-J) Top enriched Hallmark pathways identified by gene set enrichment analysis (GSEA). p-values were adjusted for multiple comparisons using FDR and Bonferroni correction. Top enriched hallmark pathways. include oxidative phosphorylation (F), interferon-γ response (G), TNFα signaling via NF-κB (H), MYC targets (I), and reactive oxygen species (J) pathways. Corresponding bar plots display log₂ fold changes of representative genes within each pathway. Blue bars indicate downregulated genes, and red bars indicate upregulated genes. Collectively, these data reveal coordinated alteration in mitochondrial metabolism, inflammatory signaling, and oxidative stress responses in the NOD1×2^−/− HSPCs.

Figure 7. — Transcriptomic profiling of donor-derived hematopoietic cells following transplantation. Bulk RNA sequencing was performed on H-2K^b donor-derived Lin- cells isolated from BALB/c recipients of WT or NOD1×2^−/− HSPCs 6 wk after stem cell transplantation (n = 3 mice/group). (Panel A) Volcano plot of differentially expressed genes comparing WT versus NOD1/2^−/−donor-derived cells, with upregulated genes shown in red and downregulated in blue. (Panel B, left) Principal component analysis (PCA) demonstrating separation of WT and NOD1/2^−/− chimera samples; (right) correlation heatmap confirming within-group clustering. (Panel C-D) Pathway enrichment analysis of differentially expressed genes. Bubble size (C) corresponds to pathway magnitude. The x-axis represents statistical significance (−log₁₀ pORA), and the y-axis shows pathway impact (−log₁₀ pAcc). The summary table (D) depicts the most significantly enriched pathways among differentially expressed genes based on over-representation and impact analyses. The table p-values, false discovery rate (FDR), and Bonferroni-adjusted p-values confirm the high statistical significance of these pathways. (Panels E-J) Top enriched Hallmark pathways identified by gene set enrichment analysis (GSEA). p-values were adjusted for multiple comparisons using FDR and Bonferroni correction. Top enriched hallmark pathways. include oxidative phosphorylation (F), interferon-γ response (G), TNFα signaling via NF-κB (H), MYC targets (I), and reactive oxygen species (J) pathways. Corresponding bar plots display log₂ fold changes of representative genes within each pathway. Blue bars indicate downregulated genes, and red bars indicate upregulated genes. Collectively, these data reveal coordinated alteration in mitochondrial metabolism, inflammatory signaling, and oxidative stress responses in the NOD1×2^−/− HSPCs.