FIG. 2.
Southern blotting analysis of the clone pBACBHV37 illustrating the insertion of the BAC vector, pBelloBACII, into the genome of the BoHV-1 strain V155. DNA was digested with either BamHI or HindIII and separated by FIGE in a 1% agarose gel. DNA fragments were transferred onto nylon membranes by Southern blotting. Duplicate membranes were hybridized with DIG-labeled probes specific for either TK of BoHV-1 or the CAP resistance gene (CAPr) of the BAC vector. (A) Southern blot hybridized with a DIG-labeled probe specific for the TK gene of BoHV-1. (B) Southern blot hybridized with a DIG-labeled probe specific for the CAPr gene. Standard molecular sizes (in base pairs) are given.