FIG. 4.

Replication kinetics of infectious BoHV-1 rescued from pBACBHV37 compared to wild-type BoHV-1 strain V155. Following recovery of infectious BoHV-1 from pBACBHV37, DNA and viral stock was amplified and titrated. CRIB-1 monolayers were infected with one TCID₅₀ of either the BoHV-1 strain V155 or pBACBHV37. The culture supernatants of each infection were recovered at 2, 4, 6, 12, 24, 48, and 72 h p.i. (PI), and the TCID₅₀ was determined for each. The titrations were done in triplicate, and averaged titers are illustrated. TCID₅₀s were determined for both extracellular and intracellular viruses.