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Schematic of on-column derivatization of a single vesicle with NDA/CN−. (A) Injection of a plug of isotonic NaCl, followed by a single vesicle, into the tapered inlet. (B) Injection of NDA/CN− into the capillary. (C) Five-minute reaction time to permit lysis of the vesicle and derivatization of its contents. (D) Electrophoresis initiated and the labeled amines separated and detected with CE-LIF.
