Table 1. Macromolecule-production information for the putative BLF1-like deamidase CWO84_RS12955.

Source organism	Methylomonas sp. Kb3
DNA source	Chemically synthesized (IDT gene, Apical)
Expression vector	2B-T
Plasmid construction method	Ligation-independent cloning (LIC)
Forward primer	5′-TACTTCCAATCCAATGCAATGACCGCAATTTCTGATTTTG-3′
Reverse primer	5′-TTATCCACTTCCAATGTTATTATTAGACATTTTTTAGCGC-3′
Expression host	E. coli strain BL21 (DE3) Star
Complete amino-acid sequence of the construct produced†	mksshhhhhhenlyfq/snaMTAISDFVANPKQFLKNNVLRVLFTMPAQPNSIGMFKFERKNYDAVKLATGANIPCYSLVPIHGQEATIFSRNATSANRDYLRAYWCPYEDDAMHSIMVGAGADFMFTSNMDGCSFGVGSATPTGDRRVAHINLRSQPNSHNLQDGTLAVQSLTDHHVKPDRYMKSSRTPGSIPGEIKATTIGIRNTATGAWSFHYQQYRLLGGQINQVYLLALKNV

^†Lower case letters indicate the vector sequence consisting of an N-terminal 6×His affinity tag and TEV protease recognition sequence introduced for recombinant expression. The slash (/) denotes the TEV protease cleavage site (ENLYFQ↓S). The affinity tag was removed by TEV protease digestion prior to crystallization, leaving three vector-derived residues (sna) at the N-terminus of the recombinant protein.